In Vitro Micropropagation of Lilium candidum Bulb by Application of Multiple Hormone Concentrations Using Plant Tissue Culture Technique

Authors

  • Akshay Milind Patil PhD Research Scholar, Centre of Biotechnology, PIMS (DU), Loni. 413736, INDIA
  • Pooja Prakash Gunjal Assistant Professor, College of Agricuiltural Biotechnology, Saralgaon Tal Murbad Dist Thane, INDIA
  • Dr. Sonali Das Director, Centre of Biotechnology, PIMS (DU), Loni-413736, INDIA

Keywords:

Micropropagation, Lilium bulb, Lilium candidum, medicinal plant, therapeutic, standardization of growth regulators

Abstract

The multiplication efficacy by bulb is low and the plantlets are more susceptible to disease, therefore, there is a need to develop a protocol for its propagation. Lilium candidum is listed in the saitma prefecture Red Data Book as a critically endangered plant and rescuing information regarding its micro-propagation is rather limited. On this regard, the application of in vitro micropropagtion procedure might help to obtain large numbers of uniform plants of endangered species of Lilium. Dried lilies are a rich source of fiber and also rich in sodium and carbs. Lily bulbs have proteins and starch and also small quantities of iron, calcium, phosphorous, and vitamin B1, B2, C. The health benefits of the lily for the heart are well known on account of the active cardiac glycosides as well as the flavonoids which tend to stimulate the arteries and can cause them to dilute. Another one of the therapeutic uses of the lily flower is in the case of treating burns and preventing the formation of scar tissue. One of the main health benefits of the lily flower is that it helps regulating the heart rate there by allowing the heart to function more efficiently and regular. Having multiple medicinal properties we decided to cultivate Lilium candidum using plant tissue culture so farming can be increased using this cost efficient techniques. In this research, we have studied various Effect of different concentration of BAP and NAA on the initiation of Lilium candidum from bulb and IBA, IAA and NAA on the rooting of shoots of Lilium Candidum.

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References

Arzate- Fernandez, A-M; Nakazaki, Tokomoto, y and Taniska,T; Efficient callus induction and plant regeneration from filament in lilium. Plant cell filaments with anther in lilium, plant cell reports,Vol-16no.12.p.836-840.

Bacchetta, Loretta; Remotti patrizio c;bernardini, clandia. Adventatious shoots regeneration from leaf explants and stems nodes of lilium .Plant cell, Tissue organ culture,July 2003, Vol.no.1,p.37-44.

Bach A And Krol . (2001). A Effect of light qualityon somatic embryogenesis in Hyacinthusorientailsliium.Biological bulletianof Poznan,Vol 36,.p-105-107.

Blom- Barnhoorn, G.J and J.VanAartijk. (1985 ). The regeneration of plants free of LSVand TBV from infected Liliumbulb-scale explants in the presence of virazole. Acta Hortic.164:163-168.

De Klerk, Geert – Jan; Delvallee, Isabelle and Paffen, Annie. (1992). Dormancy release of micropropagated bulbets Liliumspeciosum after long culture InSoil.Hort. Science, Vol.27,no.2p.147-149.

Hong –Mei, Sun ;Tian –Lai , Liand Yun- Fei, Li. (2005). Physiology Mechanism Of Metabolism In The Middle Scale Of Ilium Davidilvar.unicolour bulbs stored in at Low Temperature Dormancy-release .Agriculture Sciences in China, Vol.4.no.7,p.321-527.

Jeong, J. H. (1996). In Vitro propagation of bulb Scale section of several Korean native lilies, Act a Horticulture , B Vol ,411, p.269-276.

Robb Shelia.M. (1957). The culture of excised tissueLiliumSpeciosum. Thune Journal of Experimental Botany, Vol 8, no.3.p-348-352.

Arzate-Fernandez A M Misawa , T Shimada, T YoYonekura , K Ogawa. (2005). Genetic Diversity of Miyamauskashi- yuri (Liliumma culantum Thumb.varbukosanense). Endangered species at Mount Buko, Saitma Japan plant species Biol.

Dhar U. J Upreti, Bhatt. (2000). Micropropagation pittosporum napaulensis (DC) Rehder and Wilson – a rare, endemic Himalaya an, medicinal tree plant tissue organ. Cult 63:231-235.

Lai, M.J. (1991). Criteria and Measure for Assessing Rare and threatened Plant species in Taiwan.The Council of Agriculture, ROC, Taipei, 76pp.

Lian M., Chakrabarty D and paek KY. (2003).bulbet formation from bulbsale segments of Lilium using bioreactor system.Biologiaplantarum 46:199-202.

Murashige, T.and F.Skoog. (1992).A revised for medium for rapid growth and bioassays with Lilium tissue culture. Physiol. Plant 15: 473-479

Nhut D T. (1998). Micropropagtion of (lilylongiflorum)Viain vitro Stem Node and pseudo – bulbets culture plant cell Rep.17;913-916.

Nhut D T, B V LE S Fukai, M Tanaka, Van. (2001). Effects of Activated Charcoal, Explant size and position or Sucrose concentration on plant and shoot regeneration of Liliumlongiflorumvia young stem culture Plant growth Reg.33;59-65.

Nilmi Y.M Nakano, N Isogai. (1999). Effects of temperature and illuminating conditions on regeneration an development of bulbets in scale ultures of seven Liliumspp . J. Japan Soc, Hort,Sci 68:28.34.

Nitzsche, W.(1983). Germplasm preservation.In D.A Evans, W.R. Sharp, P.V. Ammirato, and Y.Yamada (eds.), Handbook of plant cell culture.V1. Collier Macmillan publishers, London,pp.782-805.

Novak, F.J, E, petru. (1981). Tissue culture propagation of Lilium hybrids.Sci.Hort.14:191-199

Noda S. (1987). Lily road toword the Japanese islands – Cytological view point; In ,The lilies of Japan , Species and Hybrids . Ms himizu(ed.) Seibundo Shinkosha. Tokuyo,pp:98-110.

Okazaki, K and M. Koizumi.(1995).callus formation and regeneration of some species of Lilium. Acta Hortic.392:97-106.

Person H A, K Lundquist , H Nybom. (1998). RAPD analysis of geneticvariation wwithin and among population of Turk’s–cap lily (Lliliummartagaon l.) Hereditas 128:213-220.

Stanilova, M.I., V.P. Ilcheva, and N.A. Zagorska. (1994). Morphogenetic potential and in vitromicropropagation of endangered species LeucojumaestivumL.Liliumientia Horticulture119:458-61.

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Published

2021-03-31

How to Cite

Akshay Milind Patil, Pooja Prakash Gunjal, & Dr. Sonali Das. (2021). In Vitro Micropropagation of Lilium candidum Bulb by Application of Multiple Hormone Concentrations Using Plant Tissue Culture Technique. International Journal for Research in Applied Sciences and Biotechnology, 8(2), 244–253. Retrieved from https://ijrasb.com/index.php/ijrasb/article/view/76

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