The Effect of Hibiscus sabdariffa Extracts on Pseudomonas aeruginosa Isolated from Wounds and Pus
DOI:
https://doi.org/10.31033/ijrasb.9.2.3Keywords:
Hibiscus sabdariffa, Pseudomonas aeruginosa, plant extracts, Wound and pus, GC/MSAbstract
The study aimed to evaluate the efficacy of Hibiscus sabdariffa extracts of aqueous and alcoholic extracts on Pseudomonas aeruginosa using agar well diffusion method. The study enrolled 100 wound and pus patients. The results showed that 44 isolates were Pseudomonas aeruginosa that equivalent to 44% total isolates. The Hibiscus sabdariffa extract concentrations used are (10, 25, 50,75 and 100 mg/ml). The bacterial isolates identified after been growing on blood agar and purified on MacConkey Agar using many biochemical tests. The bacterial identification was confirmed using Vitek-2 compact system technique. The results showed that the aqueous extracts were more active than alcoholic extract and no inhibition at the concentrations (10, 25 and 50 mg/ml) against Pseudomonas aeruginosa for aqueous and alcoholic extracts, the inhibition zones were (11 and 13) mm at the concentrations (75 and 100) mg/ml respectively for aqueous extract, and (10 and 11) mm at the concentrations (75 and 100) mg/ml respectively for alcoholic. Sensitivity test was conducted for Pseudomonas aeruginosa using Vitec compact test. FTIR and GC/MS tests conducted for the plant extracts and the results detailed in the study.
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References
Islam, M. M. (2021). A review on medicinal properties some commonly used culinary agents of Bangladesh. Journal of Medicinal Plants, 9(3), 111-117.
Islam, M. M. (2019). Food and medicinal values of roselle (Hibiscus sabdariffa L. Linne Malvaceae) plant parts: a review. Open J Nutr Food Sci, 1(1), 1003.
Hurtado, A. Q; Neish, I. C., & Critchley, A. T. (2019). Phyconomy: the extensive cultivation of seaweeds, their sustainability and economic value, with particular reference to important lessons to be learned and transferred from the practice of eucheumatoid farming. Phycologia, 58(5), 472-483.
Jamini, T. S., Islam, A. A., Mohi-ud-Din, M., & Saikat, M. H. (2018). Phytochemical composition of calyx extract of roselle (Hibiscus sabdariffa L.) genotypes. Journal of Agriculture and Food Sciences, 16(1), 13-23.
Jasim, A. N., & Ad’hiah, A. H. (2009). The effect of aqueous plant extracts (Hibiscus sabdariffa and Glycyrrhiza glabra), on the growth of Entamoeba histolytica in vitro. Journal of Biotechnology Research Center, 3(1), 50-55.
Wright, C. I., Van-Buren, L., Kroner, C. I., & Koning, M. M. G. (2007). Herbal medicines as diuretics: a review of the scientific evidence. Journal of ethnopharmacology, 114(1), 1-31.
Almana, H. A. (2001). Karkade, Hibiscus sabdariffa as a mineral and fiber supplement in chocolate cakes. Arab Universities Journal of Agricultural Sciences (Egypt).
Riose . A.; Jone . M. and Anderson , W ( 1987 ) , Preparation of water and alcoholic extracts in plants.J. B.O.T 15;155 -159.
Al-Kareemi, K. K. (2012). Inhibitory effect of parsley (Petroselinum crispum) juice against some urinary pathogens in vitro. The Iraqi Postgraduate Medical Journal, 11(3), 336-342.
Emwas, A. H; Luchinat, C; Turano, P; Tenori, L; Roy, R; Salek, R. M., ... & Wishart, D. S. (2015). Standardizing the experimental conditions for using urine in NMR-based metabolomic studies with a particular focus on diagnostic studies: a review. Metabolomics, 11(4), 872-894.
Clinical and Laboratory Standards Institute (CLSI) .(2020). The performance standard for antimicrobial susceptibility testing; Twenty Fourth Informational Supplement. Vol.34(1). USA.
Rajan, A; Cherian, E., & Baskar, G. (2016). Biosynthesis of zinc oxide nanoparticles using Aspergillus fumigatus JCF and its antibacterial activity. Int J Mod Sci Technol, 1, 52-7.
Nasrollahzadeh, M., Bidgoli, N. S. S., Issaabadi, Z., Ghavamifar, Z., Baran, T., & Luque, R. (2020). Hibiscus Rosasinensis L. aqueous extract-assisted valorization of lignin: preparation of magnetically reusable Pd NPs@ Fe3O4-lignin for Cr (VI) reduction and Suzuki-Miyaura reaction in eco-friendly media. International journal of biological macromolecules, 148, 265-275.
Aftan, M. M; Toma, M. A; Dalaf, A. H; Abdullah, E. Q., & Salih, H. K. (2021). Synthesis and Characterization of New Azo Dyes Based on Thiazole and Assess the Biological and Laser Efficacy for Them and Study their Dyeing Application. Egyptian Journal of Chemistry, 64(6), 2903-2911.
Sarvestani, S., Hosseini, S. M., & Farhangfar, S. H. (2020). Effect of Aqueous-Alcoholic Extract of Hibiscus sabdariffa Calyx and Leaf Calyx and Leaf on Performance, Egg Quality, Immune System and Antioxidant Balance of Laying Hens. Iranian Journal of Applied Animal Science, 10(2), 317-325.
Jabeur, I; Pereira, E; Barros, L; Calhelha, R. C; Soković, M; Oliveira, M. B. P., & Ferreira, I. C. (2017). Hibiscus sabdariffa L. as a source of nutrients, bioactive compounds and colouring agents. Food Research International, 100, 717-723.
Tegos, G; Stermitz, F. R; Lomovskaya, O., & Lewis, K. (2002). Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials. Antimicrobial agents and chemotherapy, 46(10), 3133-3141.
Jasiem, T. M., Nasser, N. M., Baderden, S. K., & Hasan, H. A. (2019). Pharmacognostical and phytochemical studies of Iraqi Hibiscus rosa-sinensis. In AIP Conference Proceedings Vol. 2144, No. 1, p. 040002. AIP Publishing LLC.
Mahasneh, A. M; Abbas, J. A., & El‐Oqlah, A. A. (1996). Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Bahrain. Phytotherapy Research, 10(3), 251-253.
Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical microbiology reviews, 12(4), 564-582.
Mickymaray, S. (2019). Efficacy and mechanism of traditional medicinal plants and bioactive compounds against clinically important pathogens. Antibiotics, 8(4), 257.
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