Assessment of Cholesteryl Ester Transfer Protein Gene Taq1B Polymorphism and Its Relationship with Lipid Levels of Hypertensive Iraqi Patients
DOI:
https://doi.org/10.31033/ijrasb.9.1.2Keywords:
Hypertension, Lipid disorders, Taq1B gene polymorphismAbstract
Lipid disorder is one of the main causes of essential hypertension. The polymorphism of cholesteryl ester transfer protein (CETP) gene was well-known to be related with the variants in the lipid profiles. Therefore, the study was targeted to determine the relationship between the polymorphism of CETP Taq1 B (rs708272) and evaluate its relationship with lipid profile levels among some hypertensive Iraqi patients. One hundred and seventy blood samples were collected from two groups, the first group included a hundred hypertensive patients and the second group included seventy healthy individuals as control group. For both groups, lipid profile was estimated, genomic DNA was extracted from all samples and Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to detect CETP gene Taq1 B polymorphism. Results indicated that the concentrations of triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL-C), and very-low-density lipoprotein (VLDL-C) had increased, whereas the concentration of high-density lipoprotein (HDL-C) was decreased in hypertensive patients in comparison to control group. The study also showed a higher Frequency for the genotype B1B1 and the (B1) allele in hypertensive patients in compare with control group (p ≤ 0.01). Lipid profile concentrations according to CETP gene Taq1 B genotypes showed non-significant differences. The conclusion of this study was indicated that the polymorphism of CETP gene Taq1B may be related with lipid disorder and CETP (B1) allele which could be used as a genetic marker for increasing hypertensive susceptibility in Iraqi population.
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Idemudia, J.O., Ugwuja, E.I. (2009) Plasma lipid profiles in hypertensive Nigerians. The Internet Journal of Cardiovascular Research.6 (2).
Bhavani, B.A., Padma, T., Sastry, B.K., Reddy, N.K. (2003). Plasma Lipoprotein (a) levels in patients with untreated essential hypertension. Indian Journal of Human Genetics. 1;9 (2).
Thompson Coon, J. (2010). Goodman and Gilman's The Pharmacological Basis of Therapeutics.12th ed New York, USA: Mc Graw Hill; p765.
Halperin, R.O., Sesso, H.D., Ma J, Buring, J.E., Stampfer, M.J., Michael Gaziano J. (2006). Dyslipidemia and the risk of incident hypertension in men. Hypertension. Am Heart Assoc. 47(1):45-50.
Borghi, C. (2002). Interactions between hypercholesterolemia and hypertension: implications for therapy. Current opinion in nephrology and hypertension: implications for therapy, Current opinion in nephrology and hypertension. LWW. 11(5):489-96.
Harvey, J.M., and Beevers, D.G. (1990). Biochemical investigation of hypertension. Annals of clinical biochemistry. SAGE Publications Sage UL: London, England. 27(4):287-96.
Appel, L.J., Moore, T.J., Obarzanek, E., Vollmer, W.M., Svetkey, L.P., Sacks, F.M., Bray, G.A., Vogt, T.M., Cutler, J.A, Windhauser, M.M., and Lin, P.H. (1997) A clinical trial of the effects of dietary patterns on blood pressure. New England Journal of Medicine. Mass Medical Soc. 336(16):1117-1124.
Knuiman, J., West, C. (1981). HDL-cholesterol in men from thirteen countries. The Lancet. Elsevier. 318(8242):367-368.
Taylor, G.O., and Agbedana, E.O. (1983). A comparative study of plasma high-density lipoprotein cholesterol in two groups of Nigerians of different socio-economic status. African journal of medicine and medical sciences. 12(1):23-28.
Gordon, T., Castelli, W.P., Hjortland, M.C., Kannel, W.B., and Dawber, T.R. (1977) High density lipoprotein as a protective factor against coronary heart disease: the Framingham Study. The American journal of medicine. Elsevier. 62(5):707-714.
Tall, A.R. (1993). Plasma cholesteryl ester transfer protein. Journal of lipid research. Citeseer. 34(8):1255-1274.
Bruce, C., and Tall, A.R. (1995). Cholesteryl ester transfer proteins, reverse cholesterol transport, and atherosclerosis. Current Opinion in Lipidology. 6(5):306-311.
Inazu, A., Brown, M.L., Hesler, C.B., Agellon, L.B., Koizumi, J., Takata, K., Maruhama, Y., Mabuchi, H., and Tall, A.R. (1990). Increased high-density lipoprotein levels caused by a common cholesteryl-ester transfer protein gene mutation. New England Journal of Medicine. Mass Medical Soc. 323(18):1234-1238.
Ordovas, J.M., Cupples, L.A., Corella, D., Otvos, J.D., Osgood, D., Martinez, A., Lahoz, C., Coltell, O., Wilson, P.W., and Schaefer, E.J. (2000). Association of cholesteryl ester transfer protein–Taq IB polymorphism with variations in lipoprotein subclasses and coronary heart disease risk: the Framingham study. Arteriosclerosis, thrombosis, and vascular biology. Am Heart Assoc. 20(5):1323-1329.
Freeman, D.J., Griffin, B.A., Holmes, A.P., Lindsay, G.M., Gaffney, D., Packard, C.J., and Shepherd, J. (1994). Regulation of plasma HDL cholesterol and subfraction distribution by genetic and environmental factors. Associations between the TaqI B RFLP in the CETP gene and smoking and obesity. Arteriosclerosis and thrombosis: a journal of vascular biology. Am Heart Assoc. 14(3):336-344.
Alharbi, K.K., Kashour, T.S, Al-Hussaini, W., Al-Nbaheen, M.S, Mohamed, S., Hasanato, R.M., Tamimi, W., Al-Naami, M.Y., and Khan, I.A. (2013). Association of angiotensin converting enzyme gene insertion/deletion polymorphism and familial hypercholesterolemia in the Saudi population. Lipids in health and disease. Springer. 12(1):177.
Hassanzadeh, T., Firoozrai, M., Zonouz, A.E., Zavarehee, A., and Paoli, M. (2009). Taq1B polymorphism of cholesteryl ester transfer protein (CETP) gene in primary combined hyperlipidaemia. Indian Journal of Medical Research. 129(3):293-298.
Liu, S., Schmitz, C., Stampfer, M.J., Sacks, F., Hennekens, C.H., Lindpaintner, K., and Ridker, P.M. (2002). A prospective study of TaqIB polymorphism in the gene coding for cholesteryl ester transfer protein and risk of myocardial infarction in middle-aged men. Atherosclerosis. Elsevier. 161(2):469-474.
Rejeb, J., Omezzine, A., Boumaiza, I., Rebhi, L., Rejeb, N.B., Nabli, N., Abdelaziz, A.B., Boughzala, E., and Bouslama, A. (2012). Four polymorphisms of cholesteryl ester transfer protein gene and coronary stenosis in a Tunisian population. Journal of Cardiovascular Medicine. LWW. 13(9):546-553.
Heidema, A.G., Feskens, E.J., Doevendans, P.A., Ruven, H.J., Van Houwelingen, H.C., Mariman, E.C., and Boer, J.M. (2007). Analysis of multiple SNPs in genetic association studies: comparison of three multi‐locus methods to prioritize and select SNPs. Genetic Epidemiology: The Official Publication of the International Genetic Epidemiology Society. Wiley Online Library. 31(8):910-921.
Horne, B.D., Camp, N.J., Anderson, J.L., Mower, C.P., Clarke, J.L., Kolek, M.J., and Carlquist, J.F., (2007). Intermountain Heart Collaborative Study Group. Multiple less common genetic variants explain the association of the cholesteryl ester transfer protein gene with coronary artery disease. Journal of the American College of Cardiology. 49(20):2053-2060.
Bruce, C., Sharp, D.S., and Tall, A.R. (1998). Relationship of HDL and coronary heart disease to a common amino acid polymorphism in the cholesteryl ester transfer protein in men with and without hypertriglyceridemia. Journal of lipid research. 39(5):1071-1078.
Naito, H.K. Kaplan, A, et al. 1984. Cholesterol. Clin Chem The C. V. Mosby Co. St Louis Toronto. Princeton. 1194-1206 and 437.
Naito, H.K. Kaplan, A, et al. 1984. HDL Cholesterol. Clin Chem The C. V. Mosby Co. St Louis Toronto. Princeton. 1207-1213 and 437.
Friedewald, W.T, Levy, R.I, and Fredrickson, D.S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry. 18(6):499-502.
Ali, S.M., Mahnaz, S., and Mahmood, T. (2008). Rapid genomic DNA extraction (RGDE). Forensic Science International: Genetics Supplement Series. Elseveier. 1(1):63-65.
Ahmed, A.I., Helal, M.M., and Kassem, K.F. (2011). Cholesteryl ester transfer protein Taq1B (g. 5454G> A) gene polymorphism in primary combined hyperlipidemia in the Egyptian population. Laboratory Medicine. Oxford University Press Oxford, UK. 42(8):482-6.
Niu, S., Tao, X. Li. J., Liu, Y., Wang, J., and Cong, M. (2017). Association of the CETP gene TaqIB and D442G polymorphisms with essential hypertension in the Chinese Mongolian population. 599–606.
Choudhury, K.N, Mainuddin, A.K., Wahiduzzaman, M., and Islam, S.M. (2014). Serum lipid profile and its association with hypertension in Bangladesh. Vascular health and risk management. 10:327.
Sarkar, D., Latif, S.A., Uddin, M.M., Aich, J., Sutradhar, S.R., Ferdousi, S., Ganguly, K.C., and Wahed, F. (2007). Studies on serum lipid profile in hypertensive patient. Mymensingh medical journal: MMJ. 16(1):70-6.
Mackness, M.I., Durrington, P.N., and Mackness, B. (2000). How high-density lipoprotein protects against the effects of lipid peroxidation. Current opinion in lipidology. LWW. 11(4):383-8.
Cai, G., Shi, G., and Huang, Z. (no date). Gender speci fi c effect of CETP rs708272 polymorphism on lipid and atherogenic index of plasma levels but not on the risk of coronary artery disease. (Ldl). 1–5.
Kalantar, Z., Reza, M., Sotoudeh, G., Djalali, M., Mansouri, A., and Alvandi, E, et al. (2017). Differences in the interaction between CETP Taq1B polymorphism and dietary fat intake on lipid pro fi le of normolipedemic and dyslipidemic patients with type 2 diabetes mellitus. Clin Nutr [Internet]. 2017; Available from: http://dx.doi.org/10.1016/j.clnu.2016.12.024
Yilmaz, H., Agachan, B., Karaali, Z.E., and Isbir, T. (2004). Taq1B polymorphism of CETP gene on lipid abnormalities in patients with type II diabetes mellitus. International journal of molecular medicine. Spandidos Publication. 13(6):889-93.
Özsait, B., Kömürcü-Bayrak, E., Poda, M., Can, G., Hergenç, G., Onat, A., Humphries, S.E., and Erginel-Ünaltuna, N. (2008). CETP TaqIB polymorphism in Turkish adults: association with dyslipidemia and metabolic syndrome. Anatolian Journal of Cardiology/Anadolu Kardiyoloji Dergisi. 8(5).
Kolovou, G., Stamatelatou, M., Anagnostopoulou, K., Kostakou, P., Kolovou, V., and Mihas, C, et al. (2010). Cholesteryl Ester Transfer Protein Gene Polymorphisms and Longevity Syndrome. Open Cardiovasc Med J. 4(1):14–9.
Schechter, C.B., Barzilai, N., Crandall, J.P., and Atzmon, G. (2010). Cholesteryl Ester Transfer Protein (CETP) genotype and reduced CETP levels associated with decreased prevalence of hypertension. Mayo Clin Proc. 85(6):522–526.
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