Evaluation of the Effect of Washing Pretreatment on The Reduction of Mesophyll and Thermophile Spores in the Apple Juice Product
DOI:
https://doi.org/10.31033/ijrasb.9.3.19Keywords:
Apple juice, the effect of peracetic acid, Spore reduction, Mesophyll, ThermophileAbstract
Juice is an important part of modern diets and drinks in many countries around the world, especially for the needy, such as the sick, the elderly, pregnant women, and children. This study aimed to investigate and compare the effect of using parasitic acid, distilled water, and 80% Tween as a detergent in removing and reducing the number of spores of mesospheric bacteria and thermophiles' in apple juice products. In this research, a quantitative method and a measuring tool have been used. The statistical population of healthy apple samples from the fields was prepared in such a way that at least 9 apples of equal size and weight are available for each repetition of the experiment. The samples were stored in the refrigerator until the experiment. Each shipment of apples was divided into three equal groups in terms of the number and weight of apples in the group and each group received the appropriate washing treatment. The results showed that the difference in 80% Tween yield in removing spores from apple surface compared to the other two treatments had a confidence level of more than 99% and the effect of peracetic acid treatment in removing mesophyll spores was not significantly different from distilled water treatment. As a result, counting mesospheric and thermophiles' spores in apple washing solution showed a significant difference in yield of 80% Tween with both other treatments and its surface activity caused its good performance in separating spores from the fruit surface compared to the other two treatments.
Downloads
References
Abdullah N, Chin NL.(2014). Application of Thermosonication Treatment in processing and production of high quality and safe-to-Drink fruit juices. Agar sci pricedia. Vol,2:320-7
Adams, M.R. A.D. Hartley, L.J. Cox. (1989).Factors affecting the efficacy of washing procedures used in the production of prepared salads. Food Microbial. Vol,6:69- 77.92
Al- Jedah JH, Robinson RK (2002). Nutritional Value and Microbiological Safety of fresh Fruit Juices, Sold through Retail Outlets in Qatar. Pakistan Jornal of Nutrition. Vol,1(2): 79-81.
Arnab, Majumdar. Neha, pradhan. Jibin, sadasivan. Ananya, Acharya . Nupur, Ojha Swathy Babu.(2019). Microbial Contamination & Food Degradation', Handbook of Food Bioengineering, Elsevier.Pp.127-130
Aguirre, C.M., Levesque, P.L., Paillet, M., Lapointe, F., St-Antoine, B.C., Desjardins, P., Martel, R (2009). The role of the oxygen/water redox couple in suppressing electron conduction in field-effect transistors. Adv. Mater Vol. 21 (30), 3087–3091.
Berger, C.N. S.V. Sodha, R.K. Shaw, P.M. Griffin, D. Pink, P. Hand and G. Frankelm.( 2010). Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ. Microbial. Vol,12(9):2385–2397.
Centers for Disease Control and Prevention (CDC), (2007). Multistate outbreak of Salmonella serotype Tennessee infections associated with peanut butter: the United States, 2006–2007. MMWR. Vol, 56 (21), 521
Deinhard, G. Blanz, P. Poralla, K. Altan, E. (1987). Bacillus acidoterrestris sp. nov.a new thermotolerant acidophile isolated from different soils. Syst. Appl. Microbiol. Vol,10, 47–53.
Groenewald, W.H. Gouws, P.A. and Witthuhn, R.C. (2009). Isolation identification and typification of Alicyclobacillus acidoterrestris and Alicyclobacillus adicadarius strains from orchard soil and the fruit processing environment in South Africa. Food Microbial. Vol, 26: 71-76.
Lateef A, Oloke JK, Gueguim-kana Eb.(2004). Antimicrobial resistance of bacterial strains isolated from orange Juice products. Afr J Biotechnol. Vol; 3(6): 334-8.
Liao, C.H. and G.M. Sapers. (2000). Attachment and growth of Salmonella Chester on apple fruits and in vivo response of attached bacteria to sanitizer treatments. J. Food Protect.Vol,63:876– 883.
Mahale DP, Khade RG,VaidyaVK (2008)..Microbiological Analysis of Street Vended Fruit Juices from Mumbai City. India. Journal of Food Safety. Vol,10:31-34.
Noël H, Hofhuis A, De Jonge R, Heuvelink AE, De Jong A, Heck ME(2010). Consumption of fresh fruit juice: how a healthy food practice caused a national outbreak of Salmonella Panama gastroenteritis. J of Foodborne Pathog Dis.Vol,7(4):375-81.
Oliveira, M. J. Usall, I. Viñas, C. Solsona and M. Abadias. (2011). Transfer of Listeria innocua from contaminated compost and irrigation water to lettuce leaves. Food Microbial. Vol, 28:590- 596.
Olorunjuwon B,Temitope B, Muibat F, Olowadn A.(2014). Microbiological quality of some locally-produced Fruit juice in Organ State, Soth Western Nigeria. J Mic Res Vol, 2 (1): 001-8.
Oita, S. Khoyama, N. (2009). Antibacterial effect of grape polyphenols against thermoacidophilic bacteria Alicyclobacillus acidoterrestris. Nippon Shokuhin Kagaku Kogaku Kaishi. Vol, 49: 555-558.
Postollec, F. Bonilla, S. Baron, F. Jan, S. Gautier, M. Mathot, A.G. Hallier-Soulier, S. Pavan, S. and Sohier, D. (2010). A multiparametric PCR-based tool for fast selection and identification of spore-forming bacteria in food. Int J Food Microbiol.Vol,142: 78-88.
Pontius, A.J. Rushing, J.E. Foegeding, P.M. (1998). Heat resistance of Alicyclobacillus acidoterrestris spores is affected by various pH values and organic acids. J. Food Prot.Vol,61: 41- 46.
Reddi S, Kumar RN, Balakrishna N, Rao vs.(2015). Microbiological quality of street vended fruit juices in Hyderabad, India and their association between food safety knowledge and practices of fruit juice vendors. Int J Curr Mic App Sci. Vol,4: 970-82.
Semit, Y. Cameron, M. Venter, P. and Witthuhn, R.C. (2011). Alicyclobacillus spoilage and isolation-A review. Food Microbiol. Vol,28:331-339.
Simforian E, Nonga H, Ndabikunze B.(2015). Assessment of microbiological quality of raw fruit juice vended in Dares Salaam city, Tanzania. Food control. Vol. 57: 302-7.
Tournas VH, HeeresJ, Burgess L (2006). Moulds and yeasts in fruit salads and fruit juices. Food Microbiology. Vol,23(7):684-88.
Ukuku, D. O. and W. Fett. (2000). The behavior of Listeria monocytogenes inoculated on cantaloupe surfaces and efficacy of washing treatments to reduce transfer from rind to fresh-cut pieces. J. Food Protect. Vol,65:924–930.
Vandekinderen, I. F. Devlieghere, B.D. Meulenaer, P. Ragaert, J. Van Camp.( 2009). Decontamination strategies for fresh-cut produce. Postharvest Rev. Vol. 4:5.
Warriner, K. A. Huber, A. Namvar, W. Fan, and K. Dunfield. (2009). Recent advances in the microbial safety of fresh fruits and vegetables. Adv. Food Nutr. Res. Vol,57:155- 179.
Wells JM, Butterfield JE (1997). Salmonella contamination associated with bacterial soft rot of fresh fruits and vegetables in the marketplace. Plant Dis. Vol, 81:867-872.
Witthuhn, R.C. van der Merwe, E. Venter, P. and Camerom, M. (2012). Guaicol Production from ferulic acid, vanillin, and vanillic acid by Alicyclobacillus acidoterrestris. Int J Food Microbiol. Vol,157: 113-117.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 International Journal for Research in Applied Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
editor@ijrasb.com 