Isolation and Diagnosis of Different Local Isolates of Saccharomyces Cerevisiae using DNA Sequencing
DOI:
https://doi.org/10.31033/ijrasb.9.3.16Keywords:
Saccharomyces cerevisiae, specific PCR, DNA sequencingAbstract
In this study, ten isolates of Saccharomyces cerevisiae were isolated from the fruit market of Mosul city. The Isolates were identified by morphological and cultural, and biochemical tests. The results showed that these isolates belong to the genus Saccharomyces cerevisiae. Performing specific PCR using primers (SC2, SC1), purified genomic DNA from local Isolates were showed eight bands at one size (1178) base pairs. The sequence of nitrogen bases for specific PCR products for DNA samples purified from three elected local isolates was determined. The results of the analysis using the DNA BLAST/NCBI program showed a similarity in the ratio (99, 98, 87) % among local isolation sequences and standard strains sequences of Saccharomyces cerevisiae recorded in the gene bank.
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