Isolation of Bacteria from Rhizosphere, Phylloplane and Caulosphere of Brinjal (Solanum melongena L.)
Keywords:
Bacteria, Rhizosphere, Phylloplane, Caulosphere, Solanum melongena LAbstract
Rhizosphere, phylloplane and caulosphere is the region where a complex community of microbes, mainly bacteria and fungi are present. The microbe- plant interaction in these regions can be beneficial, neutral, variable, or deleterious for plant growth. The bacteria that exert beneficial effects on plant development are termed plant growth promoting bacteria. To isolate the bacteria from rhizosphere, phylloplane and caulosphere of brinjal (Solanum melongena L.). The seeds of 16 cultivars of brinjal (Solanum melongena L.) viz., Arka keshav, Arka shirish, Arka kusumaker, and IIHR accession numbers 389,386,387,377 Tc, BB44, 391, 433, 434, 427, 447, 448, 476 and 487 that were used in the initial screening experiment were obtained from the Department of Vegetable crops, IIHR, Hessaraghatta, Bangalore. Brinjal (Solanum melongena L.) plants of different varieties were collected from seven locations around Bangalore viz., Hessaraghatta, Yelahanka, Kengeri, Madi vala, Hebbal, Tirumalapura and Attibele were also screened for the presence of associative bacteria. Associative microorganisms isolated from the rhizosphere, phylloplane and shoot regions of brinjal (Solanum melongena L.), revealed the presence of three morphologically different colonies. 80% of 16 cultivars of the brinjal (Solanum melongena L.) screened showed the presence of associative bacterial colonies. In this study diazotrophic BBI were obtained from the rhizoplane, phylloplane and stem of 16 cultivars of brinjal (Solanum melongena L.) that were screened. The dominant pearl-colored colonies isolated from all varieties of brinjal plants that were screened was identified and showed maximum nitrogen fixing ability compared with that of the other colonies. The phylloplane of brinjal (Solanum melongena L.) plants from seven different locations around Bangalore showed the presence of the dominant pearl-colored colonies. Moderate growth of bacteria was observed in root, stem and leaf bits sterilized up to 35 minutes. Even on surface sterilized roots which were homogenized and inoculated on growth media, dense growth of bacteria was observed there by establishing the presence of bacteria inside the root system. For the first time the presence of growth promoting bacteria on the rhizosphere and endorhizosphere of brinjal (Solanum Melongena L.) cultivars was established.
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References
Dobereiner, J. (1980). Forage grasses and grain crops. Methods for evaluating biological nitrogen fixation, 535-536.
Hill, W. A., Bacon-Hill, P., Crossman, S. M., & Stevens, C. (1983). Characterization of N2-fixing bacteria associated with sweet potato roots. Canadian journal of microbiology, 29(8), 860-862.
Nair, S. K., & Rao, N. S. (1977). Microbiology of the root region of coconut and cacao under mixed cropping. Plant and Soil, 46(3), 511-519.
McClung, C. R., van Berkum, P., Davis, R. E., & Sloger, C. (1983). Enumeration and localization of N2-fixing bacteria associated with roots of Spartina alterniflora Loisel. Applied and Environmental Microbiology, 45(6), 1914-1920.
Graciolli, L. A. (1981). Microorganisms in the phyllosphere and rhizosphere of sugarcane. Associative N_2 Fixation, 91-101.
Pohlman, A. A., & McColl, J. G. (1982). Nitrogen fixation in the rhizosphere and rhizoplane of barley. Plant and Soil, 69(3), 341-352.
Dart, P. J., & Wani, S. P. (1982). Non-symbiotic nitrogen fixation and soil fertility.
Rao, N. S. (1983). Nitrogen-fixing bacteria associated with plantation and orchard plants. Canadian journal of microbiology, 29(8), 863-866.
Klucas, R. V., Pedersen, W., Shearman, R. C., & Wood, L. V. (1981). Nitrogen fixation associated with winter wheat, sorghum, and Kentucky bluegrass [Triticum aestivum, sorghum bicolor, Poa pratensis].
Kumari, M. L., Kavimandan, S. K., & Subba Rao, N. S. (1970). Occurrence of nitrogen fixing Spirillum in roots of rice, sorghum, maize & other plants. Indian Journal of Experimental Biology, 14(5), 638-639.
Patriquin, D. G., & Döbereiner, J. (1978). Light microscopy observations of tetrazolium-reducing bacteria in the endorhizosphere of maize and other grasses in Brazil. Canadian journal of microbiology, 24(6), 734-742.
Patriquin, D. G., Döbereiner, J., & Jain, D. K. (1983). Sites and processes of association between diazotrophs and grasses. Canadian Journal of Microbiology, 29(8), 900-915.
Bashan, Y., & Holguin, G. (1986). Anchoring of Azospirillum brasilense to hydrophobic polystyrene and wheat roots. Microbiology, 139(2), 379-385.
Bashan, Y., Levanony, H., & Whitmoyer, R. E. (1991). Root surface colonization of non-cereal crop plants by pleomorphic Azospirillum brasilense Cd. Microbiology, 137(1), 187-196.
Quimio, A. J., & Coroza, A. G. (1986). Plant growth promoting activity of rhizobacteria on tomato, white potato and corn [Philippines]. Supplement No. 1.
Quimio AJ, Cadapan ET. Field performance of selected plant growth promoting rhizobacteria (PGPR) on corn, sorghum and cowpea. Philippine Journal of Crop Science. 1988.
Sukhada M. (1987). Field response of tomato (Lycopersicon esculentum Mill ‘Pusa Ruby’) to inoculation with a VA mycorrhizal fungus Glomus fasiculatum and with Azotobacter vinelandii. Plant and soil, 98(2), 295-297.
Bashan, Y. (1983). Enhancement of wheat root colonization and plant development by Azospirillum brasilense Cd. following temporary depression of rhizosphere microflora. Applied and Environmental Microbiology, 51(5), 1067-1071.
Bashan, Y., Harrison, S. K., & Whitmoyer, R. E. (1990). Enhanced growth of wheat and soybean plants inoculated with Azospirillum brasilense is not necessarily due to general enhancement of mineral uptake. Applied and Environmental Microbiology, 56(3), 769-775.
Boddey, R. M., & Dobereiner, J. (1988). Nitrogen fixation associated with grasses and cereals: recent results and perspectives for future research. Plant and soil, 108(1), 53-65.
Lalande, R., Bissonnette, N., Coutlee, D. and Antoun, H. (1989). Plant and Soil (Netherlands).;117 (2): 207-218.
Brand, I., Lugtenberg, B. J. J., Glandorf, D. C. M., Bakker, P. A. H. M., Schippers, B., & De Weger, L. A. (1991). Isolation and characterization of a superior potato root-colonizing Pseudomonas strain. Bulletin OILB SROP (France).
Ahn, S. B., Gamo, H., & Yuk, C. S. (1990). Isolation of N 2-fixing Microorganism from the Root of Non-leguminous Crops. Korean Journal of Soil Science and Fertilizer, 23(1), 67-72.
Tyler, M. E., Milam, J. R., Smith, R. L., Schank, S. C., & Zuberer, D. A. (1979). Isolation of Azospirillum from diverse geographic regions. Canadian Journal of Microbiology, 25(6), 693-697.
Malik, K. A., & Bilal, R. (1989). Survival and colonization of inoculated bacteria in kallar grass rhizosphere and quantification of N 2-fixation. In Nitrogen Fixation with Non-Legumes (pp. 301-310). Springer, Dordrecht.
Bilal, R., Rasul, G., Arshad, M., & Malik, K. A. (1993). Attachment, colonization and proliferation of Azospirillum brasilense and Enterobacter spp. on root surface of grasses. World Journal of Microbiology and Biotechnology, 9(1), 63-69.
Agarwala-Dutt, R., Tilak, K. V. B. R., & Rana, J. P. S. (1991). Isolation of Azospirillum from the interior of various parts of some graminaceous plants. Zentralblatt für Mikrobiologie, 146(3), 217-219.
Lukin, S., Kozhevin, P., & Zviagintsev, D. (1990). Spatial distribution of Azospirillum brasilense cells in the rhizosphere of barley plants. Mikrobiologiâ (Moskva, 1932), (6), 1090-1093.
Holguin, G., Guzman, M. A., & Bashan, Y. (1992). Two new nitrogen-fixing bacteria from the rhizosphere of mangrove trees: Their isolation, identification and in vitro interaction with rhizosphere Staphylococcus sp. FEMS Microbiology Letters, 101(3), 207-216.
Dalton, H. (1980). The cultivation of diazotrophic microorganisms. The cultivation of diazotrophic microorganisms., 13-64.
Gamo, T. (1990). Azospirillum spp. from crop roots: a promoter of plant growth. JARQ. Japan Agricultural Research Quarterly, 24(4), 253-259.
Gamo, T., & Ahn, S. B. (1991). Growth-promoting Azospirillum spp. isolated from the roots of several non-gramineous crops in Japan. Soil Science and Plant Nutrition, 37(3), 455-461.
Bashan, Y., Singh, M., & Levanony, H. (1989). Contribution of Azospirillum brasilense Cd to growth of tomato seedlings is not through nitrogen fixation. Canadian Journal of Botany, 67(8), 2429-2434.
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