Paper Chromatographic Separation of Plant Pigments a New Approach by using Akshya-Swagatika Solvent and Mobile Chromatogram Detection System (MCDS)

Abstract

Chromatography is a term that refers to a group of laboratory techniques for separating mixtures. Chromatography works on the premise of solute partitioning between two phases or solvents. The technique of paper chromatography is commonly used to separate plant pigments based on their molecular weight. Plant pigments include chlorophyll-b, chlorophyll-a, carotenoid, and xanthophyll, which all have various molecular weights, colours, and absorption maxima. In this study, an attempt was made to see how a new solvent (Akshya-Swagatika solvent) can be used to separate plant pigments using paper chromatography, as well as a new detection method developed by us known as Mobile chromatogram detection system (MCDS) that can be used for compound identification and photographing. As shown in the table and photograph, pigments are separated by molecular weight and band colour, with carotenoid having the highest RF value and chlorophyll-b having the lowest. It was obvious that the Akshya-Swagatika solution could be used to separate plant pigments in paper chromatography. Photograph taken with a mobile chromatogram detection system that is much clearer than normal one. In developing countries, both solvent and detection systems are useful in explaining paper chromatography in a cost-effective manner. The MCDS detection system is cost-effective fast method which replaces traditional sophisticated detection procedures.