Screening for the Production and Characterization of L-asparaginase from Endophytic Fungi
Keywords:
L-asparaginase, fungal endophytes, acute lymphoblastic leukemiaAbstract
L-asparaginase (EC 3.5.11. L-asparagine amidohydrolase) is first enzyme, studied very intensively in human beings with regard to its anti-tumor potential against tumor of lymphoid precursor, acute lymphoblastic leukemia (ALL). The current drugs are suffering from many side effects like immune suppression, infertility, secondary neoplasm. The immunogenic complications associated with its present microbial sources Escherichia coli; Erwinia carotovora limits its medicinal frontier. So there exists a need of switching to novel natural sources to serve as non-immunogenic and better production sources of L-asparaginase. In the present study, four cultures of fungal endophytes viz. TSF-1, TSF-2, TSF-3 and TSF-4 selected on the basis of primary and secondary screening was carried on with L-asparagine as a sole carbon and nitrogen source and phenol red as pH indicator. The maximum protein content was observed to be present in TSF-2 i.e. 2.727 mg /mL and possessed maximum activity of 6.054 Units/ml. Sample was separated by SDS-PAGE, stained by silver staining, showed a single band with molecular weight of approximately ~45kDa.
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Abbas Ahmed M.M, Dahab, N.A, Taha T, and Hassan, F (2015). Production, purification and characterization of L-Asparaginase from Marine Endophytic Aspergillus sp. ALAA-2000 under Submerged and Solid State Fermentation. J. Microb. Biochem. Technol, 7(3):165-172.
Ali S.S. (1994). A fungal L-Asparaginase with potential antitumour activity; Ind., J. Microbiol., 34: 73-76.4455.
Dura M.A, Flores, M, and Toldra, F (2002). Purification and Characterization of L-Glutaminase from Debaryomyces spp. Int. J. Food. Microbial. 76: 117-126.
El-Bessoumy A.A., Sarhan M., Mansour J. (2004). Production, isolation, and purification of L-Asparaginase from Pseudomonas aeruginosa 50071 using solid-state fermentation. J. Biochem Mol Biol., 37: 387-393.
Gulati R., Saxena R.K., Gupta R. (1997). A rapid plate assay for screening L-asparaginase producing micro-organisms. Lett Appl Microbiol; 24: 23-26.
Iwasa T, Fujii M, Yokotsuka T (1987). Glutaminase produced by Cryptococcus albidus ATTCC 20293. Purification and some properties of the enzyme. Nippon shoyu kenkyusho Zasshi. 13: 205-210.
Kumar K. and Verma N., (2012). The various sources and application of L-Asparaginase, Asian J. Biochem. Pharma. Res., 3: 197-205.
Kusari S., Hertweck C. and Spiteller M. (2012). Chemical Ecology of Endophytic Fungi: Origins of Secondary Metabolities. Chemistry & Biology. 19: 792-798.
Lonsane, B.K., N.P. Ghilyal, S. Budiatman and S.V. Ramakrishnan, 1995. Engineering aspects of solid state fermentation, Enzyme Microb. Technol., 7: 228-257.
Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein Measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
Meghavarnam A. K. and Janakiraman S. (2015). A simple and efficient dye based technique for rapid screening of fungi for L-Asparaginase production. Journal of experimental biology and agriculture sciences. 3(2): 124-130.
Nagarethinam S., Nagappa A.N., Udupa N., Venketa Rao J., Meenashi Vanathi B (2012). Microbial L-Asparaginase and its future prospects. Asian Journal of Medical Research. 1: (4): 159-168.
Narta U.K., Kanwar S.S. and Azmi W. (2007). Pharmacological and clinical evaluation of L-Asparaginase in the treatment of Leukemia. Crit. Rev. Oncology Hematology, 61: 208-221.
Patil M.P., Patil R.H. and Maheshwari V.L. (2012). A novel and sensitive agar plug assay for screening of asparaginase-producing endophytic fungi from Agele marmelos. Acta Biologica Szegrediensis. 56(2):175-177.
Pandey, A.G., Snakacs, C.R. Soccol, A. Jose, Rodriguez-Leon and V.T. Soccol, 2001. Production, Purification and properties of microbial phytases. Bioresource Technolo., 77: 203.
Sindhwad P. and Desai K. (2015). Media Optimization, Isolation and Purification of L-Asparaginase from Marine Isolate. Asian Pac. J. Health Sci., 2(3):72-82.
Strobel G.A. (2003). Endophytes as sources of bioactive products. Microbes and Infection. 5: 535-544.
Yasser R, Abdel-Fattah, Zakia A (2002) L-Asparaginase production by Pseudomonas aeruginosa in solid-state culture: Evaluation and optimization of culture conditions using factorial designs. Prospects in Biochem. 38: 115-122.
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