Molecular Screening of bla-TEM-68 Gene for TEM-68 ESBLs Resistant K. pneumoniae and C. freundii from Hospital Waste in Adamawa state Specialist Hospital, Yola
Keywords:
bla-TEM-68 gene, Enterobacteriaceae, K. pneumoniae, C. freundiiAbstract
Resistant strains of K. pneumoniae and C. freundii due to under use and over use of antibiotics and biocides has contributed to economic burden due to nosocomial infections. This study focuses on screening hospital wastes for antibacterial and biocide resistant K. pneumoniae and C. freundii in Adamawa state specialist hospital, Yola. One hundred and twenty (120) hospital wastes samples were analysed. Samples were cultured on appropriate media and incubated at 37 oC for 24h. Biochemically identified isolates were molecularly confirmed and sequenced. K. pneumoniae being 93% identical to strain KPNIH48 and C. freundii being 99% identical to complex sp CFNH4 were confirmed. Resistant K. pneumonia and C. freundii were subjected to phenotypic ESBLs test, then DDS test was carried out to confirm ESBLs production. Phenotypically ESBLs positive K. pneumoniae were positive for DDST but negative for C. freundii. The DDST positive isolates were then screened for bla-TEM-68 gene. All the DDST positive K. pneumoniae harboured the bla-TEM-68 gene but none in C. freundii. The isolates carrying bla-TEM-68 gene were cured using 10 % SDS and the results showed that the ESBLs genes were plasmid and/or chromosomally encoded. The presence of K. pneumoniae carrying bla-TEM-68 gene indicates a possibility for increased nosocomial infections in Adamawa state and Nigeria at large and so relevant preventive recommendations were made.
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References
World Health Organization (1999). Unsafe injection practices and transmission of blood borne pathogens. Bull. World Health Organization.77:787-819
Baquero, F., Martinez, J.L. and Canton, R. (2008). Antibiotics and antibiotic resistance in water environments. Current Opinion in Biotechnology. 19:260-5
Program for Appropriate Technology in Health (PATH), (2009). Achieving effective sharp waste management in GAVI host countries. A proposed approach with estimates of cost 2006. Available at http:/www.path.org. Accessed 23rd march, 2016.
Bailey, J.K., Pinyon, J.L., Anantham, S. and Hall, R.M. (2010). Commensal Eschirichia coli of healthy humans: a reservoir for antibiotic-resistance determinants. Journal of Medical Microbiology. 59, 1331-1339
Szmolka, A. and Nagy, B. (2013). Multidrug resistant commensal Escherichia coli in animals and its impact for public health. Frontiers in Microbiology 4:258.
Alanis, A.J. (2005). Antibiotics susceptibility pattern of Enterobacteriaceae isolated from hospitals. Archive of Medical Research. 36, 697
Washington State Department of Health (2014). CDC guidlines and recommendations on the surveillance and control and carbapenem resistant enterobacteriaceae. p 420-97.
World Health Organization (2002). W.H.O Report on the emerging new cases of antibacterial resistance in Gram-negative bacteria.
World Health Organization (2018). Epidemiology of Antibacterial resistance: global report on surveillance. Geneva: World Health Organization.
World Health Organization (2014). Antibacterial resistance: global report on surveillance. Geneva: World Health Organization.
Neidell, M.J., Cohen, B., Furuya, Y, Hill, J., Jeon, C.Y., Glied, S. and Larson, E.L. (2012). Costs of healthcare‐ and community‐associated infections with antibacterial‐resistant versus antibacterial‐susceptible organisms. Clinical Infectious Diseases 55: 807–815.
Martin‐Loeches, I, Torres, A., Rinaudo, M., Terraneo, S., de Rosa, F., Ramirez, P., Diaz, E, Fernandez‐Barat, L., Li Bassi, G.L and Ferrer, M. (2015). Resistance patterns and outcomes in intensive care unit (ICU)‐acquired pneumonia. Validation of European Centre for Disease Prevention and Control (ECDC) and the Centers for Disease Control and Prevention (CDC) classification of multidrug resistant organisms. Journal of Infections, 70: 213-222.
Lim, C.J., Cheng, A.C., Kong, D.C and Peleg, A.Y. (2014). Community‐onset bloodstream infection with multidrug‐resistant organisms: a matched case‐control study. Biomed Central Infectious Diseases 14: 126
Stefaniuk, E., Suchocka, U., Bosacka, K and Hryniewicz, W. (2016). Etiology and antibiotic susceptibility of bacterial pathogens responsible for community‐acquired urinary tract infections in Poland. European Journal of Clinical Microbiology and Infectious Diseases, 35: 1363–1369.
Pandey, S. (2006). Weter pollution and health-review articles. Kathmandu University Medical Journal 4:128-13.
Sosa, A.J, de, J., Byarugaba, D.K., Amabile, C., Hsueh, P.R., Kariuki, S. and Okeke, I.N. (Eds). (2010). Antibacterial Resistance in developing countries. New York: ISBN: 978-0-38789369-3. Springer Science, 23:554.
Russo, T.A. and Johnson J.R. (2003). Medical and Economic impact of Extra-intestinal infections due to Escherichia coli: focus on an increasingly important endemic problem. Microbes and Infections 5:449-456.
World Health Organization (2002). W.H.O Report on the emerging new cases of antibacterial resistance in Gram-negative bacteria.
Shears, P. (2000). Atimicrobial resistance in the tropics. Tropical Doctors. 30:114- 116
Shears, P. (2001). Antibiotic resistance in the tropics. Epidemiology and surveillance of Antibacterial resistance in the Tropics. Trans. R. Soc. Journal of Tropical Medicine and Hygiene, 95:127-130
Howard, D., Cordell, R., McGown, J.E. Jnr., Packard, R.M. and Scott, R.D.II. and Solomon, S.L (2001). Measuring the economic costs of antibacterial resistance in hospital settings: the summary of the Centre for Desease Control and Prevention-Emory workshop. Clinical Infectious Diseases 33:1573-1578.
Paladino, J.A, Joseph, A., SunderlinConnie, S., Price, J. and Schentag, J. (2002). Economic consequences of antibacterial resistance. Surgical Infections Reviews (Larchmt) 3:3.
Cosgrove, S.E. and Carmeli, Y. (2003). The impact of antibacterial resistance on health and economic outcomes. Journal of Clinical Infectious Disease; 36:1433-1437.
Byarugaba, D.K. (2004). A view on antibacterial resistance in developing Countries and responsible risk factors. International Journal of Antibacterial Agents 24(2):105-10
Parimi, N., Pinto Pereira L.M. and Prabhakar, P. (2002). The general public perceptions and the use of antibacterials Trinidad and Tobago. Pan American Journal of Public Health 12:11-18.
Lipstitch, M, and Samoure, M.H. (2002). Antibacterial use and antibacterial resistance: a population perspective. Emerging Infectious Diseases. 8:347-354
Hossain, M.M., Glass, R.I. and Khan, M.R (1982). Antibiotic use in a rural community in Bangladesh. International Journal of Epidemiology 11:402- 405.
Kumarasamy, K. K., Toleman, M. A and Walsh, T. R. (2010). Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. The Lancet Infectious Diseases 10(9):597-602.
Bush K., Jacoby, G. A. and Medeiros A. A (1995). A functional classification scheme for β-lactamases and its correlation with molecular structure. Antibacterial Agents and Chemotherapy. 1995; 39(6):1211–1233
Queenan, A. M and Bush K. (2007). Carbapenemases: the versatile β-lactamases. Clinical Microbiology Reviews. 20(3):440–458.
Kim, P.W., Harris, A.D., Roghmann, M.C., Morris, J.G. Jnr., Strinvasan, A. and Perencevich, E.N. (2003). Epidemiologic risk factors for isolation of ceftri- axone-resistant versus-susceptible C. freundii in hospitalized patients. Antibacterial Agents in Chemotherapy 47(9): 2882:2887.
Nada, T., Baba, H., Kawamura, K., Ohkura, T., Torii, K. and Ohta, M. (2004). A small outbreak of third generation cephalosporin-resistant Citrobacter freundii infection on surgical ward. Japanese Journal of Infectious Diseases. 57, 181-182.
Chuang, Y., Tseng, S., Teng, L., Ho, Y and Hsueh, P. (2006). Emergence of cefotaxime resistance in Citrobactor freundii causing necrotizing fasciitis and osteomyelitis" Journal of Infections 53:161-163.
Ciğerci, I.H., Korcan, S.E., Konuk., M and Oztürk, S. (2007). Comparison of ALAD activities of Citrobacter and Pseudomonas strains and their usage as biomarker for Pb contamination. Environmental Monitoring and Assessment 139(1-3):41-8.
Walsh, C. (2003) Antibiotics: action, origins, resistance Washington DC. ASM press.
Bradford, P.A (2001). Extended spectrum beta-lactamases in the 21st century: Characterization, epidemiology and detection of the important resistance Threat. Clinical microbiology Reviews 14:933-951
Paterson, D.L. and Bonomo, R.A. (2005). Extended spectrum beta-lactamase-producing Escherichia coli causing bacteremia in centralized Canadian Region. Journal of Clinical Microbiology 50(2):294-9.
Poirel, K., Bonnin, R.A. and Nordman, P. (2012). Genetic support and diversity of acquired extended-spectrum beta-lactamase in Gram-negative rods. Infection Genetics and Evolution Journal 12(5):883-93
Kitzis, M.D., Billlout- Klein, D., Goldstein, F.W., Williamson, R., Tran Van Nhieu, G., Carlet J., Acar, J.F. and Gutmann, L. (1988). Dissemination of the novel Plasmid-mediated beta-lactamase CTX-1, which confers resistance to Broad - spectrum cephalosporins, and its inhibition by beta-lactamase inhibitors. Antibacterial Agents and Chemotherapy 32, 9-14.
Radice, M., Power, P. Di Conza, J. and Gebriel, G. (2002). Early, dissemination of CTX-M- derived enzymes in south America. Antimicrob. Agents Chemother 46:602-604
Canton, R. and Coque, T.M. (2006). The CTX-M beta-lactamase pandemic. Current Opinion in Microbiology. 9:466-475.
Bonnet, R. (2004). Growing group of extended spectrum beta-lactamases: the CTX-M enzymes. Antibacterial Agents and chemotherapy. 48:4-14
Pitout, J.D. (2010). Infections with extended-spectrum Beta-Lactamase-Producing enterbacteriaceae: Changing epidemiology and drugs treatment choices. Drugs journal 70(3):313-33
Arpin, C., Dubbois V. Maugein, J., Jullin, J., Dutilh, B., Brochet, J., Larribet, G., Fischer, I. and Quentin, C (2005). Clinical and molecular analysis of extended spectrum beta-lactamase-producing enterobacteria in the community setting. Journal of Clinical Microbiology. 43:5048-5054
Lewis, J.S., Herrera, M., Wickes, B., Patterson, J.E. and Jorgensen, J.H. (2007). First report of the Emergence of CTX-M-type extended – spectrum beta – lactamase (ESBLSs) as the predominant ESBLS isolated in a U.S. Health care system. Antibacterial Agents and Chemotheapyr 51, 4015-4021.
Pitout, J.D. and Laupland, K.B. (2005). Extended-spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public health concern. Lancet infectious Diseases 8:159-166.
Wieler, L.H., Ewers, C. Guenthers, S, Walther, B. and Lubke-Becker. A. (2011). Methicilin-resistant staphylococci (MRS) and extended-spectrum-beta lactamases (ESBL)-producing Enterobacteriaceae in companion animals: nosocomial infections are one reason for the rising prevalence of these potential zoonotic pathogens in clinical samples. International Journal of Medical Microbiology, 301:635-641.
Mshana, S.E., Imirzalioglu, C., Hamid, H.C., Hein, T., Dormann, E. and Chakraborty, T (2009). Conjugative IncFI plasmid carrying CTX-M-15 among Escherichia coli ESBLS producing isolates at a university hospital in Germany. Biomed Central Infecious Diseases 9:97.
Livermore, D.M., Canton, R., Gniadkowski, M, Nordmann, P., Rossolini, G.M., Alert, G, Ayala, J., Coque, T.M., Kern-ZdNanowicz, I., Luuzzaro, F., Poirel, L.L. and Woodford, N. (2007). CTX-M: changing the face of ESBLs in European Journal of Antibacterial Chemotherapy, 59, 165-174.
Adebayo, A.A. & Tukur, A.L. (1999). Adamawa State in maps. First edition. Paraclete Publishers, Yola.
American Public Health Association (APHA) (1999). Standard methods for examination of water and waste water, Washington. American Public Health Association (APHA), America water works Association (AWWA), Water Environment Federation (WEF). pp 1120
Fawole, M.O. and Oso, B.A. (2001). Laboratory manual of Microbiology. Spectrum books L.t.d. p77.
Cheesbrough, M. (2006). District Laboratory Practice in Tropical countries. 2nd Edition, Cambridge University Press, Cambridge, UK., ISBN-13: pp 46
Schreckenberger, P.C., Janda, J.M., Wong, J.D. and Barone, E.J. (1999). Algorithm for identification of Gram-negative bacteria. In: Manual of clinical Microbiology. Washington: ASM press. pp. 438-441.
Engelkirk, P.G., and Duben-Engelkirk, J. (2008). Laboratory diagnosis of Infectious Diseases. Essentials of Diagnostic Microbiology. Balti Lippincott William and Wilkins.
Trindade, L.C., Marques, E., Lopes, Lopes, D.B. and Ferreira, M.A.S.V (2007). Development of a molecular method for detection and identification of Xanthomonas compestris, Plasmopara viticola. The Official Journal of Sao Paulo State Plant Pathology Association, 33(1):16-23.
Mark, G. D. and Murrell, C.J. (2005). Stable isotope probing-linking microbial identity to function. Nature Review Microbiology 3(6), 499.
Frank, J.A., Reich, C.I., Sharma, S., Weisbaum, J.S., Wilson, B.A and Olsen, G.J. (2008) Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA Genes. Applied and Environmental Microbiology. Vol. 74. pp. 2461-2470
www.appliedbiosystems.com accessed on 29th June, 2019.
Paterson, D. L., Hujer, K. M., Hujer, A. M. Yeiser, B, Bonomo, M. D, Rice, L. B. and Bonomo, R.A. (2003). Extended Spectrum Beta-Lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV- and CTX-M-type -lactamases. Antibacterial Agents and Chemotheapy 47:3554–3560.
Khanfar, H.S., Bindayna, K.M., Senok, A.C. and Botta, G.A. (2009). Extended spectrum beta-lactamase (ESBLS) in Escherichia coli and Klebsiella pneumoniae: trend in the hospital and community settings. Journal of infection in Developing countries 3(4):295-299.
Jarosz, D. F., Godoy, V.G., Delaney, J.C., Essigmann, J.M. and Walker, G.C. (2009). A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-familiy DNA polymerase. Proceedings of the National Academy of Science United States of America. 106, 21137-21142.
Wright, G.D. (2005). Bacterial resistance to antibiotics: enzymatic degradation and modification. Advanced Drug Delivery Reviews, 57:1451-1470.
Babic, M., Hujer, A.M. and Bonomo, R.A. (2006). What’s new in antibiotic resistance? Focus on beta-lactamases. Drug Resistance update 9:142-156
Drawz, S.M. and Bonomo, R.A (2010). Review of three decades of beta-lactamase inhibition. Clinical Microbiology Review 23(1):160-201
Faith, M., Villaneuva, E., Thelma, D., Tupaci, G., Abiad, M.D., Benida Q., Baello, C., Rowa, C. and Crnado, D. (2003). Extended spectrum beta-lactamase production among Escherichia coli and Klebsiella spp. Philippines Journal Microbiological Infectious Diseases 32: 3,103-108.
Meatherall, B.L., Gregson, D., Ross, T., Pitout, J.D. and Laupland, K.B. (2009). Incidence, risk factors, and outcomes of Klebsiella pneumomiae bacteria. American Journal of Medicine 122(9):866-873
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