In-Vitro Antioxidant Activity of Total Phenolic and Flavonoid Content of Solanum nigrum Leaf
DOI:
https://doi.org/10.31033/ijrasb.9.2.12Keywords:
Flavonoids, Phenols, in-vitro, solvents, Solanum nigrumAbstract
Solanum nigrum belonging to the family Solanaceae, has been considered as a remedy for treating various ailments like epilepsy, gastric ulcers etc. and is believed to have hepatoprotective activities. In this study, the total phenolic and flavonoid content of the extracts of Ethyl acetate and Methanol from the Solanum nigrum leaves and their antioxidant activity was carried out by using spectrophotometric methods. Methanol extract with high absorbance exhibited highest antioxidant activity containing highest quantity of phenolics and flavonoids followed by Ethyl Acetate. The presence of phenolic and flavonoid compounds indicated that these compounds can contribute to the antioxidant activity. These active constituents alone or in combination with others may be responsible for the observed antioxidant activity. The findings indicated promising antioxidant activity of crude extracts of leaves needs further exploration for their effective use in both modern and traditional system of medicines. The Solanum nigrum leaf can be regarded as promising candidate for natural plant sources of antioxidants with higher values. A comparative study of the total phenolic and flavonoid contents extracted by the solvents, and their in-vitro antioxidant activity that could be an effective remedy in hepatoprotection was carried out in the study.
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Akhtar MS, Munir MJ (1989). Evaluation of gastric anti-ulcerogenic effects of Solanum nigrum, Brassica oleracea and Ocimumbasilicum in rats. Ethnopharmacology, 27: 163.
Adil Munir, Bushra Sultana, Tanveer Babar, Asad Bashir, Muhammad Amjad, Qadeer ul Hassan. Investigation on the Antioxidant Activity of Leaves, Fruit and Stem Bark of Dharik (Melia azedarach). European Journal of Applied Sciences, 2012; 4(2):47-51.
Arcy WG (1991) The Solanaceae since 1976 with a review of its biogeography. In: Solanaceae III: Taxonomy, Chemistry and Evolution. Academic press, London, pp:75-137.
Benavente-Garcia O, Castillo J, Marin FR, Ortuno A, Del-Rio JA (1997). Uses and properties of Citrus flavonoids. J.Agric. Food Chem. 45: 4505-4515
Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical biochemistry, 44(1), 276-287.
Farhan H, Rammal H, Hijazi A, Hamad H, Daher A, Reda M. In vitro antioxidant activity of ethanolic and aqueous extracts from crude Malva parviflora grown in Lebanon. Asian J Pharm Clin Res. 2012; 5(3):234-238.
Ferreira ICFR, Baptista P, Vilas-Boas, M and Barros L: Free-radical scavenging capacity and reducing power of wild edible mushrooms from Northeast Portugal: individual cap and stipe activity. Food Chemistry 2007, 100(4): 1511-1516.
Huang DJ, Ou BX, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53: 1841-1856.
Jamuna S, Paulsamy S, Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaerisradicata L. (Asteraceae), Journal of Applied Pharmaceutical Science. 2012; 02(07):149-154.
McCune LM, Johns T (2002). Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous people of North American boreal forest. J. Ethnopharmacol. 82: 197-205.
Meyer AS, Isaksen A (1995). Application of enzymes as food antioxidants. Trends Food Sci. Tech. 6: 300-304.
Mohsen SM and Ammar ASM, Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chem.2009, 112:595-598.
Maryam Zahin, Farrukh Aqil and Iqbal Ahmad. The in-vitro Antioxidant Activity and Total Phenolic Content of Four Indian Medicinal Plants. International Journal of Pharmacy and Pharmaceutical Sciences, 2009; 1(1):88-95.
Maurya S. and Singh D. (2010) ‘Quantitative Analysis of Total Phenolic Content in Adhatoda Vasica Nees Extracts’ International Journal of Pharmtech Research; Vol. 4: pp:- 2403-2406.
Oktay M, Gulcin I, Kufrevioglu OI (2003). Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. Leb. Wissen. Technol. 36: 263-271.
Piaxao, N., R. Perestrelo, J. C. Marques and J. S. Camara.2007. Relationship between antioxidant capacity and total phenolic content of red, rose and white wines. Food Chemistry 105: 204-214.
Pourmorad F., Hosseinimehr S.J. and Shahabimajid N. (2006), Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants, African Journal of Biotechnology, Vol.5 (11), Page No. 1142-19.
Prior, R., Wu, X. & Schaich, K. Standardized M Lopez A, Rico M, Rivero A and de Tangil MS: The effects of solvents on the phenolic contents and antioxidant activity of Stypocaulonscoparium algae extracts. Food Chem.2011, 125: 1104-11
Prior, R., Wu, X. & Schaich, K. Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements. J. Agric. Food Chem. 53, 4290-4302 (2005).
Rice-Evans C, Miller N, Paganga G, (1997). Antioxidant properties of phenolic compounds. Trends Plant Sci. 2: 152-159.
Sahreen S, Khan MR and Khan RA: Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food Chem.2010, 122: 1205-1211ethods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements. J. Agric. Food Chem. 53, 4290-4302 (2005).
Savitree M., Isara P., Nittaya S.L. and Worapan S. (2004), Radical Scavenging Activity and Total Phenolic Content of Medicinal Plants Used in Primary Health Care, Journal of Pharm Sci., Vol.9(1), Page No. 32-35.
Saran Kumar Gupta, Mitali Ghosala, Rajib Biswasb, Biman Krishna Sahab, Abhaya Prasad Dasb, Palash Mandala. Evaluation of in vitro antioxidant activity of methanolic extracts of some ferns from Mawsynram of Meghalaya, India, INT J CURR SCI. 2014, 12:87-97.
Spigno G, Tramelli L and de Faveri DM: Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. J. Food Eng.2007, 81:200-208
Shimada K, Fujikawa K, Yahara K and Nakamura T: Antioxidative properties of xanthan on the autooxidation of soyabean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry 1992, 406: 945-948.
Sharififar F, NudehDehgun G and Mirtajaldini M: Major flavonoid with antioxidant activity from Teucrium polium L. Food Chem.2008, 112:885-888.
Singleton, V. L., Rossi Jr., J. A. & Rossi J A Jr. Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Am. J. Enol. Vitic. 16, 144-158 (1965).
Yen GC, Chen HY (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem. 43: 27-32.
Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF and Bilaloglu V: Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf ex DC), sage (Salvia triloba L.), and Black tea (Camelliasinensis) extracts. Journal of Agricultural and Food Chemistry 2000, 48,10: 5030-5034.
Yasantha Athukorala, Kil-Nam Kin, You-Jin Jeon (2006), Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food and chemical toxicology. 44(7):1065-74.
Zengin H.W., Aktumsek A., Guler G.O., Cakmak Y.S., and Yildiztugay E. (2011) ‘Antioxidant properties of methanolic extract and fatty acid composition of centaureaurvillei dc. Subsp., rec.’ Nat. Prod.; Vol. 5(2): 123-132.
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