The Co-Existence of Cryptosporidium parvum with Others Parasitic Protozoan Infection Among Children Under Five Years in Some Places of Mosul City
DOI:
https://doi.org/10.31033/ijrasb.9.2.1Keywords:
Cryptosporidium Parvum, Chronic diarrhea, Entamoeba histolytica, Giardia lambilia, Modified Zeihl Neelsen staining, ELISAAbstract
A hospital based, cross sectional study this was carried out at the period between the beginning of June 2020 to the end of September 2021. A total of 600 child Suffering from chronic diarrhea were examined. The co-existence of mixed infection of Cryptosporidium Parvum with others intestinal parasitic protozoan parasites as an etiology of chronic diarrhea was the aim of this study, Cryptosporidium Parvum, Entamoeba histolytica, Giardia lambilia was found to be the more common parasites as a cause of chronic diarrhea among children under five years, stool samples were collected form children who lived in some places of Mosul city which is divided into two sides as a result of the passage of the Tigris River from the center, a total of 400 (66.6%) positive cases were recorded, the highest infectious rate was found at the Right side of Mosul city 230 (38.3%) cases while the rest 170 (28.3%) cases was recorded in Left side. Direct slide preparation method with a Modified Zeihl Neelsen, cultivation of Protozoal parasites by using special media and ELISA method for stool samples were used to perform the diagnosis of infection of Cryptosporidium Parvum, Entamoeba histolytica and Giardia lambilia that was to be found in 130 (21.6%),150(25%),120(20%), respectively, The co-existence of mixed infection of Cryptosporidium Parvum with Entamoeba histolytica was recorded in 30 (5%)cases while with Giardia lambilia was found in 24(4%)cases these study also recorded the presence of mixed infection between Entamoeba histolytica and Giardia lambilia in 12(2%) cases.
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References
WHO. Reading on diarrhea. Geneva :WHO publications,1992:147.
Jomaladda-P.R.Bat-R V. Parastic continalim of stored water used for drinking cooking in Hyderabad Top-Med – public healt. 1995, 214):789.94
AL-Najar - S.A. Mukhlis - FAOdislan SM. Talir-R.M Intestinal parasites and rotavirus in diarrhea. J.Fac-Med Ragledad 2000.422) 210-214
Higuera, A., Villamizar, X., Herrera, G., Giraldo, J. C., Vasquez-A, L. R., Urbano, P. & Ramírez, J. D. (2020). Molecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombia. PeerJ, 8, e8554.
Aniesona, A. T., & Bamaiyi, P. H. (2014). Retrospective Study of Cryptosporidiosis Among Diarrhoeic Children in the Arid Region of North‐Eastern N igeria. Zoonoses and public health, 61(6), 420-426.
Fayer, R., (2010). Taxonomy and species delimitation in Cryptosporidium. Exp. Parasitol.,124:90-97.
Mahmoudi, MR, Ongerth, J.E, Karannis, P. Cryptosporidium and cryptosporidiosis :the Asian perspective. lnt J Hyg Environ Health. 2017;220:1098-1109 .
Platts-Mills, J. A., Babji, S., Bodhidatta, L., Gratz, J., Haque, R., Havt, A.& MAL-ED Network Investigators. (2015). Pathogen-specific burdens of community diarrhoea in developing countries: a multisite birth cohort study (MAL-ED). The Lancet Global Health, 3(9), e564-e575.
Khalil, I. A., Troeger, C., Rao, P. C., Blacker, B. F., Brown, A., Brewer, T. G. et al.(2018). Morbidity, Mortality, and Long-Term Consequences Associated With Diarrhoea From Cryptosporidium Infection in Children Younger Than 5 Years: A Meta-Analyses Study. Lancet Glob Health 6(7),e758-e768. doi: 10.1016/S2214- 109X(18)30283-3.
Feng, Y., Ryan, U. M., and Xiao, L. (2018) . Genetic diversity and population structure of Cryptosporidium . Trends Parasitol. 34,997-1011. doi : 10.106/j.p.t.2018.07.009.
Kotloff, K. (2017). The burden and etiology of diarrheal illness indeveloping countries. Pediatric Clinics of North America 64,799-814.
Kotloff, K. L., Nasrin, D., Blackwelder, W. C., Wu, Y., Farag, T., Panchalingham, S. & Levine, M. M. (2019). The incidence, aetiology, and adverse clinical consequences of less severe diarrhoeal episodes among infants and children residing in lowincome and middle-income countries: a 12-month case-control study as a follow-on to the Global Enteric Multicenter Study (GEMS). The Lancet Global Health, 7(5), e568-e584
Bordes, L., Houert, P., Costa, D., Favennec, L., Vial-Novella, C., Fidelle, F.& Razakandrainibe, R. (2020). Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum. Parasite, 27.
Monge, S., Pijnacker, R., van Pelt, W., Franz, E., Kortbeek, L. M., & Mangen, M. J. J. (2019). Accounting for long-term manifestations of Cryptosporidium spp. infection in burden of disease and cost- of-illness estimations, the Netherlands (2013–2017). Plos one, 14(3), e0213752.
Ehsan, M. A., Akter, M., Ahammed, M., Ali, M. A., & Ahmed, M. U. (2016). Prevalence and clinical importance of Cryptosporidium and Giardia in human and animals. Bangladesh Journal of Veterinary Medicine, 14(2), 109-122
Liu, S., Roellig, D. M., Guo, Y., Li, N., Frace, M. A., Tang, K., ... & Xiao, L. (2016). Evolution of mitosome metabolism and invasion related proteins in Cryptosporidium. BMC genomics, 17(1), 1-16
Garcia - LS Bruckner DA Diagnostic Medical parasitology 2nd ed U.S.A American society for microbiology1991 :6-20.
Christie –A.B. Giardiasis Epidemiology, pathophysiology and Treatment. Postgraduate doctor. 1981,3(6):216-21.
Ober huber-G, Kastner -N . Stolte M. Giardiasis: a histological analysis of 567 cases. Scend - J - Gastroenterol. 1997, 32(1):48-51.
Hardin J.A Buret -A6. Olson M.E. Kimm M.H. Gall-D.G. Mast cell hyperplasia and increase macromolecular uptake in an animal model of giardiasis J-parasiul 1992,83(5): 908-912
Herniksen - S.A. Poble L. Sting of Cryptosporidium by a Modified Ziehl Nelson technique. Acta - Vet-Soard - 1981.22: 59490
Al-Idrrisi, S.R.; AL-Azizz, S.A.A.; AL-Emara, G.Y.; AL-Badran, A. SH (2008) Culture of Entamoeba histolytica in vitro. ALQadisiya Journal of Veterinary Medicine Sciences. 7(1):1-6
Potters, I. & Van Esbroeck, M. (2010). Negative staining technique of Heine for the detection of Cryptosporidium spp.: a fast and simple screening technique. The Open Parasitology Journal, 4(1).
Younis, Shireen H. Alkhashab, Firas M. B. (2021) Epidemiological study of Cryptosporidium parvum parasite in some districts of Nineveh province, with referring to histopathological effects in lab mice. BERJ_Volume 17_Issue 4_Pages 1023-1048.
Salman, Yahya Jirjees , Wajdi Sabeh Sadek, Zaynab Khorsheed Rasheed.(2015).Prevalence of Cryptosporidium parvum a mong Iraqi displaced people in Kirkuk city using direct microscopy, flotation technique and ELISA – copro antigen test.Int. J.Curr. Microbiol. App.Sci(2015) 4 (11): 559-572.
Tamomh, A. G., Agena, A. M., Elamin, E., Suliman, M. A., Elmadani, M., Omara, A. B., & Musa, S. A. (2021). Prevalence of Cryptosporidiosis among children with diarrhea under five years admitted to Kosti teaching hospital, Kosti City, Sudan. BMC Infectious Diseases, 21(1), 1-6.
Jarad, N. I. (2020). Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province. Iraqi Journal of Veterinary Sciences, 34(2), 441-445.
Izadi, S., Mohaghegh, M. A., Ghayour-Najafabadi, Z., Azami, M., Mirzaei, F., Namdar, F. & Hejazi, S. H. (2020). Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16. Iranian journal of parasitology, 15(1), 31.
Murray, R., Tataryn, J., Pintar, K., & Thomas, M. K. (2017). Estimates of the burden of illness for eight enteric pathogens associated with animal contact in Canada. Epidemiology & Infection, 145(16), 3413-3423.
El- Helalya NS, Alyb MM, Attiab SS. (2012). Detection of Cryptosporidium infection among children with diarrhea. New York Science Journal; 5: 68- 76.
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